蛋白质纯化实验指南——用于蛋白质组学研究（影印版）图书简介
蛋白质纯化实验指南——用于蛋白质组学研究（影印版）
蛋白质纯化实验指南——用于蛋白质组学研究（影印版）
蛋白质纯化实验指南——用于蛋白质组学研究（影印版）
蛋白质纯化实验指南——用于蛋白质组学研究（影印版）:

内容简介
蛋白质组学研究的第一步是要把蛋白质从复杂的大分子混合物中分离纯化出来。就此，本书提供了全面的背景资料、理论、实验方案和实验结果的优化方法，以及疑难解答，包括专门用于蛋白质组学研究的制备、色谱层析和电泳技术，用于结构蛋白质组学研究的蛋白质纯化分析技术等。本书是冷泉港实验室出版社最新推出的Purifying Proteins for Proteomics:A Laboratory Manual的英文影印版，是《蛋白质与蛋白质组学实验指南》的姐妹篇，秉承了冷泉港实验手册一贯的先进性、实用性和权威性，内容丰富，信息全面，方案设计完善，技术适用范围较广。
本书是遗传学、分子生物学研究者从基因组学、基因型研究转向蛋白质组和表型研究的必备工具书，适于生物化学、分子生物学、细胞生物学、遗传学、免疫学、蛋白质组学、功能基因组学等生命科学相关领域研究院所、高校相关院系、实验室的教师、研究生、科研人员，以及生物技术企业的研发者和决策者参考使用。

目录
Preface
Purifying Proteins for Proteomics Companion Web Site
Section Ⅰ:Preparing Proteins for Proteomic Analysis
1 Role of Separation Science in Proteomics
2 Strategies for Purifying Proteins
Section Ⅱ:Chromatographic Methods for Preparing Proteins for Proteomics
3 Introduction to Chromatographic Methods for Protein and Peptide Purification
4 Multidimensional Chromatography of intact Proteins
5 High-throughput Screening of Soluble Recombinant Proteins
6 lon-exchange Chromatography
7 Size-exclusion Chromatography
8 Role of Reversed-phase High-performance Liquid Chromatography in Protein Isolation and Purification
9 Hydrophobic Interaction Chromatography
10 Affinity and Immunoaffinity Chromatography
11 Dye-Ligand Affinity Chromatography in Proteomic Research
12 Immobilized Metal-ion Affinity Chromatography
13 Protein Chromatography on Hydroxyapatite
14 Chromatofocusing
Section Ⅲ:Electrophoretic Methods for Preparing Proteins for Proteomics
15 Introduction to Strategies Based on Electrophoresis for Separating Proteins
16 Separation of Proteins Using Two-dimensional Gel Electrophoresis
17 Sample Preparation for High-resolution Two-dimensional Electrophoresis by Isoelectric Fractionation in a MCE
18 An Electrophoresis Method for Purifying Proteins:The Gradiflow BF400 Instrument
19 Protein Purification Using Free-flow Electrophoresis
Section Ⅳ:Analysis of Purified Proteins Prior to Structural Proteomics
20 Introduction to Methods for Characterizing Purified Proteins
21 Characterization of Proteins by Mass Spectrometry
22 Analytical Ultracentrifugation:Protein Size,Conformation,and Interactions
23 Measuring the Conformational Stability of a Protein
24 Combining Surface Plasmon Resonance with Mass Spectrometry:ldentifying Binding Partners and Characterizing Interactions
25 Analysis of Carbohydrate from Glycoproteins
Appendices
1.An Overview of Protein Structure
2.Measuring the Concentration of Proteins
3.Concentrating Solutions of Proteins
4.Stabilization of Proteins for Storage
5.One-dimensional Polyacrylamide Gel Electrophoresis
6.Troubleshooting Guides
7.Cautions
Index

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